Comparative study of maggot (Hermetia illucens) oil extraction using mechanical pressing and soxhlet methods: Physicochemical characteristics

Yuni Marsella, RR Dirgarini Julia Nurlianti Subagyono, Chairul Saleh, Rudy Agung Nugroho, Saibun Sitorus, Teguh Wirawan, Aman Sentosa Panggabean

Abstract


Larvae of Hermetia illucens have been increasingly recognized as a promising renewable lipid feedstock for various industrial, oleochemical, and biotechnological applications. This study compared mechanical pressing and Soxhlet extraction methods to evaluate their effects on the physicochemical properties and lipid composition of maggot oil. Oil extracted by the Soxhlet method resulted in a higher yield (34.60%) than mechanical pressing (22.45%), indicating more efficient lipid recovery. The moisture contents of the oils were 0.643% and 0.468%, respectively. Both extraction methods produced oils with low acid values (1.693 ± 0.127–1.702 ± 0.116 mg KOH/g) and free fatty acid contents (0.603 ± 0.045–0.607 ± 0.042 %), suggesting acceptable oil quality. Saponification values ranged from 157 to 159 mg KOH/g, while peroxide values were 13.56–14.49 meq O₂/kg. The elemental composition results revealed that both oils were predominantly composed of carbon and hydrogen, reflecting their lipid-rich nature. Gas chromatography–mass spectrometry analysis revealed that the extraction method significantly influenced lipid composition. Mechanical pressing preserved a higher proportion of triglycerides, whereas Soxhlet extraction recovered larger amounts of free fatty acids, especially those with medium- and long-chain structures. These findings indicate that extraction techniques not only affect oil yield but also influence the physicochemical characteristics and chemical profile of H. illucens oil, which may influence its suitability for different biological and biotechnological applications. The results demonstrate that selecting a suitable extraction method is crucial for producing maggot oil with characteristics tailored to specific downstream applications, including biofuel, oleochemical, and biotechnological products.


Keywords


Hermetia illucens; maggot oil; mechanical pressing; physicochemical properties; Soxhlet extraction

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References


Abbasi, I. (2025). Integrated thermocatalytic and photocatalytic valorization of Hermetia illucens biomass for renewable fuel and chemical production. Cleaner and Circular Bioeconomy, 12, 100168. https://doi.org/10.1016/j.clcb.2025.100168

Almeida, C., Murta, D., Nunes, R., Baby, A, R., Fernandes, A., Barros, L., Rijo, P., & Rosado, C. (2022). Characterization of lipid extracts from the Hermetia illucens larvae and their bioactivities for potential use as pharmaceutical and cosmetic ingredients. Heliyon, 8(5), e09455. https://doi.org/10.1016/j.heliyon.2022.e09455

Amrul, N. F., Kabir Ahmad, I., Ahmad Basri, N. E., Suja, F., Abdul Jalil, N. A., & Azman, N. A. (2022). A review of organic waste treatment using Black Soldier Fly (Hermetia illucens). Sustainability, 14(8), 4565. https://doi.org/10.3390/su14084565

Amaryllis, A. R., Aflah, A. T., Vera, N., Erwanto, Y., Utama, D. T., & Abidin, M. Z. (2024). Quality and oxidative stability of tallow extracted by dry- and wet-rendering. Indonesian Food Science and Technology Journal, 8(1), 36–42. https://doi.org/10.22437/ifstj.v8i1.32528

AOAC International. (2023). Official Methods of Analysis of AOAC INTERNATIONAL (22nd ed.). Official Method 985.19: (Apparent) Weight per Unit Volume and Specific Gravity of Fats and Oils: Pycnometer Method. Oxford University Press. https://doi.org/10.1093/9780197610145.003.3491

Arachchige, A. M. M., Williams, M., Fernando, W. M. A. D. B., Karnpanit, W., Lumanlan, J., & Jayasena, V. (2025). The impact of solvent extraction and enzyme-assisted extraction on the yield and quality of black soldier fly larvae (Hermetia illucens) oil. International

Journal of Food Science and Technology, 60(1), 1-10. https://doi.org/10.1093/ijfood/vvaf088

ASTM International. (2016). ASTM D5291-16: Standard test methods for instrumental determination of carbon, hydrogen, and nitrogen in petroleum products and lubricants. ASTM International.

Atabani, A. E., Silitonga, A. S., Badruddin, I. A., Mahlia, T. M. I., Masjuki, H. H., & Mekhilef, S. (2012). A comprehensive review on biodiesel as an alternative energy resource and its characteristics. Renewable and Sustainable Energy Reviews, 16(4), 2070–2093. https://doi.org/10.1016/j.rser.2012.01.003

Azmir, J., Zaidul, I. S. M., Rahman, M. M., Sharif, K. M., Mohamed, A., Sahena, F., Jahurul, M. H. A., Ghafoor, K., Norulaini, N. A. N., & Omar, A. K. M. (2013). Techniques for extraction of bioactive compounds from plant materials: A review. Journal of Food Engineering, 117, 426–436. https://doi.org/10.1016/j.jfoodeng.2013.01.014

Bao, Y., & Pignitter, M. (2023). Mechanisms of lipid oxidation in water-in-oil emulsions and oxidomics-guided discovery of targeted protective approaches. Comprehensive Reviews in Food Science and Food Safety, 22(4), 2678–2705. https://doi.org/10.1111/1541-4337.13158

Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37, 911–917. https://doi.org/10.1139/O59-099

Caligiani, A., Marseglia, A., Leni, G., Baldassarre, S., Maistrello, L., & Dossena, A. (2018). Composition of black soldier fly prepupae and systematic approaches for the extraction of lipids, proteins, and chitin. Food Research International, 109, 488. https://doi.org/10.1016/j.foodres.2017.12.012

Camara-Ruiz, M., Sánchez-Venegas, A., Blasco-Lavilla, N., Hernández M. D., Sánchez-Liarte, F., Fernández-Gutiérrez, D., & Lara-Guillén A. J., (2023). Comparative assessment of insect processing technologies for sustainable insect protein production. Sustainability, 15, 13735. https://doi.org/10.3390/su151813735

Chew, Z. L., Kua, Y. L., Mah, J. K., Ra, N., Lee, T. Z. E., & Yap, S. K. C. (2025). Temperature-controlled mechanical pressing of black soldier fly larvae (BSFL): Yields and qualities of oil and protein meal. Journal of the American Oil Chemists’ Society, 102(10), 1501–1512. https://doi.org/10.1002/aocs.70007

Chia, S. Y., Tanga, C. M., Khamis, F. M., Mohamed, S. A., Salifu, D., Sevgan, S., Fiaboe, K. K. M., Niassy, S., van Loon, J. J. A., & Dicke, M. (2018). Threshold temperatures and thermal requirements of black soldier fly (Hermetia illucens): Implications for mass production. PLOS ONE, 13(11). https://doi.org/10.1371/journal.pone.0206097

Demirbas, A. (2004). Combustion characteristics of different biomass fuels. Progress in Energy and Combustion Science, 30(2), 219–230. https://doi.org/10.1016/j.pecs.2003.10.004

Demirbaş, A. (2008). Progress and recent trends in biofuels. Progress in Energy and Combustion Science, 33(1), 1–18. https://doi.org/10.1016/j.pecs.2006.06.001

Fitria, M., Alvita, L.R., Putra, F.A., Variyana, Y., & Hanifah, W., (2025). Analysis of process variables effecton the efficiency of soxhletation extraction of larvae oil (Hermetica illucens) using response surface methodology. Indonesian Journal of Chemical Analysis. 8(1). https://doi.org/10.20885/ijca.vol8.iss1.art7

Forfang, K., Zimmermann, B., Kosa, G., Kohler, A., & Shapaval, V. (2017). FTIR spectroscopy for evaluation and monitoring of lipid extraction efficiency for oleaginous fungi. PLOS One, 12(1). https://doi.org/10.1371/journal.pone.0170611

Fornari, T., Vázquez, L., Villanueva-Bermejo, D., Hurtado-Ribeira, R., Hernández, D. M., & Martin, D. (2023). Effect of moisture and oil content in the supercritical CO₂ defatting of Hermetia illucens larvae. Foods, 12(3), 490. https://doi.org/10.3390/foods12030490

Franco, a., Scieuzo, C.,Salvia, R., Petrone, A.M., Tafi, E., Moretta, A., Schmitt, E., & Falabella, P., (2021), Lipids from Hermetia illucens, an Innovative and Sustainable Source, Sustainability, 13, 10198. https://doi.org/10.3390/su131810198

Gunstone, F. D. (Ed.). (2011). Vegetable oils in food technology: Composition, properties and uses (2nd ed.). Wiley-Blackwell. https://doi.org/10.1002/9781444339925

International Organization for Standardization. (1983). ISO 660:1983 Animal and vegetable fats and oils—Determination of acid value and of acidity. Geneva, Switzerland: ISO.

International Organization for Standardization. (2020). ISO 3657:2020 Animal and vegetable fats and oils—Determination of saponification value. Geneva, Switzerland: ISO.

Ismail, N., Kamal, M. F. M., & Ya, K., (2020), Antioxidant activities of threadfin bream (Nemipterus japonicus) hydrolysate and its effect on oxidative stability of frying oil. Scientific Research Journal. 17(2), 129-148. https://doi.org/10.24191/srj.v17i2.7707

Ishak S., Kamari A., Yusoff S., & Halim A. (2018). Optimisation of biodiesel production of Black Soldier Fly larvae rearing on restaurant kitchen waste, Journal of Physics: Conf. Series, 1097, 012052. https://doi.org/10.1088/1742-6596/1097/1/012052?utm

Kim, T.-K., Lee, J.-H., Yong, H. I., Kang, M, C., Cha, J. Y., Chun, J. Y., & Choi, Y, S. (2022). Effects of defatting methods on the physicochemical properties of proteins extracted from Hermetia illucens larvae. Foods, 11(10), 1400. https://doi.org/10.3390/foods11101400

Knothe, G. (2005). Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Processing Technology, 86(10), 1059–1070. https://doi.org/10.1016/j.fuproc.2004.11.002

Knothe, G., & Steidley, K. R. (2005). Kinematic viscosity of biodiesel fuel components and related compounds. Fuel, 84(9), 1059–1065. https://doi.org/10.1016/j.fuel.2005.01.016

Knothe, G., & Razon, L. F. (2017). Biodiesel fuels. Progress in Energy and Combustion Science, 58, 36–59. https://doi.org/10.1016/j.pecs.2016.08.001

Kumar, M., Singh, V. K., Ravi, R., Verma, V., Arora, A., Alam, T., Yadav, A., & Sharma, A. (2024). Biodiesel production from microalgae oils: A critical review. Proceedings of the Institution of Mechanical Engineers, Part A: Journal of Power and Energy, 240(2). https://doi.org/10.1177/09544089241251775

Laroche, M., Perreault, V., Marciniak, A., Gravel, A., Chamberland, J., & Doyen, A. (2019). Comparison of conventional and sustainable lipid extraction methods for the production of oil and protein isolate from edible insect meal. Foods, 8(11), 572. https://doi.org/10.3390/foods8110572

Lawal, K. G., Kavle, R. R., Akanbi, T. O., Mirosa, M., & Agyei, D. (2022). Lipid nutritional indices, regioisomeric distribution, and thermal properties of Tenebrio molitor and Hermetia illucens larvae fat. Journal of Asia-Pacific Entomology, 25(3), 101951. https://doi.org/10.1016/j.aspen.2022.101951

Li, X., Dong, Y., Sun, Q., Tan, X., You, C., Huang, Y., & Zhou, M. (2022). Growth and fatty acid composition of Black Soldier Fly Hermetia illucens (Diptera: Stratiomyidae) larvae are influenced by dietary fat sources and levels. Animals, 12(4), 486. https://doi.org/10.3390/ani12040486

Li, Q., Zheng, L., Qiu, N., Cai, H., Tomberlin, J. K., & Yu, Z. (2011). Bioconversion of dairy manure by Hermetia illucens larvae: Reduction of waste and biodiesel production. Waste Management, 31(6), 1316–1320. https://doi.org/10.1016/j.wasman.2011.01.005

Lin, C, Y., & Ma, L. (2022). Effects of water removal from palm oil reactant by electrolysis on the fuel properties of biodiesel. Processes, 10(1), 115. https://doi.org/10.3390/pr10010115

Maanari, C. P., Gugule, S., Fatimah, F., Utami, A. R. P., Mustapa, M., Rumengan, S. M., Jannah, M., Akbar, J. S., & Rintjap, D. S. (2023). Effect of potassium hydroxide concentration as a catalyst on the yield of coconut oil (Cocos nucifera) transesterification. Indonesian Journal of Pure and Applied Chemistry, 6(3), 142–150. https://doi.org/10.26418/indonesian.v6i3.65513

Mai, H. C., Dao, N. D., Lam, T. D., Nguyen, B. V., Nguyen, D. C., & Bach, L. G. (2019). Purification process, physicochemical properties, and fatty acid composition of Black Soldier Fly (Hermetia illucens Linnaeus) larvae oil. Journal of the American Oil Chemists' Society, 96(11), 1303–1311. https://doi.org/10.1002/aocs.12263

Makkar, H. P. S., Tran, G., Heuzé, V., & Ankers, P. (2014). State-of-the-art on use of insects as animal feed. Animal Feed Science and Technology, 197, 1–33. https://doi.org/10.1016/j.anifeedsci.2014.07.008

Meher, L. C., Vidya Sagar, D., & Naik, S. N. (2006). Technical aspects of biodiesel production by transesterification—A review. Renewable and Sustainable Energy Reviews, 10(3), 248–268. https://doi.org/10.1016/j.rser.2004.09.002

Mohamed, H., Marusich, E., Afanasev, Y., & Leonov, S. (2021). Fatty acids-enriched fractions of Hermetia illucens (Black soldier fly) larvae fat can combat MDR pathogenic fish bacteria Aeromonas spp. International Journal of Molecular Sciences, 22(16), 8829. https://doi.org/10.3390/ijms22168829

Monita, L., Sutjahjo, S, H., Amin, A, A., Rini, M., & Fahmi. (2017), Urban organic waste processing using black soldier fly larvae (Hermetia illucens). Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan. 7(3), 227-234. https://doi.org/10.29244/jpsl.7.3.227-234

Muangrata, R., & Pannasai, S. (2024). Exploring the potential of black soldier fly larvae oil: Supercritical CO2 extraction, physicochemical analysis, antioxidant properties, shelf life, and keratinocyte growth inhibition. Journal of Agriculture and Food Research, 15, 101008. https://doi.org/10.1016/j.jafr.2024.101008

Nugroho R, A., Aryani R., Manurung H., Sari W., Sanjaya A, S., Suprihanto D., & Rudianto, P, W. (2022). Maggot dan lalat tentara hitam. PT Insan Cendekia Mandiri Group.

Pietri, P., Georgiopoulos, G., & Stefanadis, C. (2021). The prognostic role of triglycerides should be revisited. Journal of the American College of Cardiology, 77(14), 1840–1841. https://doi.org/10.1016/j.jacc.2020.12.068

Purbaningtias, T. E., & Sandy. (2024). Evaluation of ethanol grade on the robustness of acid number determination in fish oil. Indonesian Journal of Chemical Analysis, 7(2), 237–244. https://doi.org/10.20885/ijca.vol7.iss2.art11

Rehman, K, U., Hollah, C., Wiesotzki, K., Rehman, R, U., Rehman, A, U., Zhang, J., Zheng, L., Nienaber, T., Heinz, V., & Aganovic, K. (2023). Black soldier fly, Hermetia illucens as a potential innovative and environmentally friendly tool for organic waste management: A mini-review. Waste Management & Research, 41(1), 81–97. https://doi.org/10.1177/0734242X221105441

Rozali, N, S., Zainuddin, N, A., Yatim, S, R., & Rasdi, W, N. (2022). Study on the extraction of black soldier fly larvae oil. Journal of Sustainability Science and Management, 17(12), 49–66. https://doi.org/10.46754/jssm.2022.12.006

Rusdi, H.O., Kusumaningrum, I. K., Nareswari, T.J., Fauziah, P. N., Maharani, R. N., & Natasya, S. (2024). Separation and determination of free fatty acids in corn oil and palm oil by liquid-liquid extraction and acidi-alkalimetric titration, Walisongo. Journal of Chemistry, 7(1), 098-106. https://doi.org/10.21580/wjc.v7i1.20461

Shahidi, F., & Zhong, Y. (2010). Lipid oxidation and improving the oxidative stability. Chemical Society Reviews, 39, 4067–4079. https://doi.org/10.1039/B922183M

Sheng, C., & Azevedo, J. L. T. (2005). Estimating the higher heating value of biomass fuels from basic analysis data. Biomass and Bioenergy, 28(5), 499–507. https://doi.org/10.1016/j.biombioe.2004.11.008

Spranghers, T., Ottoboni, M., Klootwijk, C., Ovyn, A., Deboosere, S., De Meulenaer, B., Michiels, J., Eeckhout, M., De Clercq, P., & De Smet, S. (2017). Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture, 97(8), 2594–2600. https://doi.org/10.1002/jsfa.8081

Srisuksai, K., Limudomporn, P., Kovitvadhi, U., Thongsuwan, K., Imaram, W., Lertchaiyongphanit, R, S, T., Kovitvadhi, A., & Fungfua, W. (2024). Physicochemical properties and fatty acid profile of oil extracted from black soldier fly larvae (Hermetia illucens). Journal of Sustainable Science and Management, Veterinary World 17(3), 518-526. https://doi.org/10.14202/vetworld.2024.518-526

Sui, H., Shao, J., Agblevor, F. A., Zhang, Y., Wang, X., Yang, H., & Chen, H. (2023). Fractional condensation and aging of pyrolysis oil from cotton stalk. Biomass and Bioenergy, 174, 106837. https://doi.org/10.1016/j.biombioe.2023.106837

Surendra, K. C., Olivier, R., Tomberlin, J. K., Jha, R., & Khanal, S. K. (2016). Bioconversion of organic wastes into biodiesel and animal feed via insect farming: A review. Renewable Energy, 98, 197–212. https://doi.org/10.1016/j.renene.2016.03.022

Suryati, T., Julaeha, E., Farabi, K., Ambarsari, H., & Hidayat, T. (2023). Lauric acid from the black soldier fly (Hermetia illucens) and its potential applications. Sustainability, 15(13). https://doi.org/10.3390/su151310383

Tzompa-Sosa, D. A., Yi, L., van Valenberg, H. J. F., van Boekel, M. A. J. S., & Lakemond, C. M. M. (2014). Insect lipid profile: aqueous versus organic solvent-based extraction methods. Journal of Insect Science, 62, 1087-1094. https://doi.org/10.1016/j.foodres.2014.05.052

Umeh, S. I., & Okonkwo, P. A. (2025). The essential properties of oils for biodiesel production. In Biodiesel plants – fueling the sustainable outlooks. IntechOpen. https://doi.org/10.5772/intechopen.1008694

Van Huis, A., Van Itterbeeck, J., Klunder, H., Mertens, E., Halloran, A., Muir, G., & Vantomme, P. (2013). Edible insects: future prospects for food and feed security. FAO Forestry.

Van Huis, A. (2020). Insects as food and feed, a new emerging agricultural sector: A review. Journal of Insects as Food and Feed, 6(1), 27–44. https://doi.org/10.3920/JIFF2019.0017

Wang, Y.-S., & Shelomi, M. (2017). Review of black soldier fly (Hermetia illucens) as animal feed and human food. Foods, 6(10), 91. https://doi.org/10.3390/foods6100091

Zhuang, Y., Dong, J., He, X., Wang, J., Li, C., Dong, L., Zhang Y., Zhou, X., Wang, H., Yi, Y., & Wang, S. (2022). Impact of heating temperature and fatty acid type on the formation of lipid oxidation products during thermal processing. Frontiers in Nutrition, 9, 913297. https://doi.org/10.3389/fnut.2022.913297




DOI: http://dx.doi.org/10.30821/biolokus.v9i1.5737

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