Formulation of Jakaba fertilizer from banana peel, pineapple peel, tea dregs for enhanced pak choi (Brassica rapa subsp. Chinensis) growth

Salwa Zainum Muttaqin, Jendri Mamangkey, Marina Silalahi, Lucas William Mendes

Abstract


Excessive use of chemical fertilizers can lead to environmental degradation and increase the financial burden on farmers. As a sustainable alternative, liquid organic fertilizer (LOF) derived from Jakaba (also known as jamur keberuntungan abadi) was fermented using common organic wastes. This study aimed to evaluate the effects of different organic waste substrates on the characteristics of Jakaba-based LOF (JLOF) and the growth performance of pak choi (Brassica rapa subsp. Chinensis). A completely randomized design (CRD) was used, consisting of nine treatments with three replications each. The concentration variations of banana peel waste, pineapple peel, and tea grounds used in fermentation include 100 g, 200 g, and 300 g. Observations were conducted in three time intervals on days 0, 14, and 30. Parameters measured included the physical and biological characteristics of the LOF (i.e., pH and fungal growth), as well as plant height, leaf number, and fresh weight of pak choi. The data obtained were analyzed statistically using ANOVA analysis. The results showed that tea dregs provided the most favorable conditions for Jakaba growth and maintained the most stable pH. In contrast, pineapple and banana peels inhibited fungal growth, likely due to the presence of inhibitory compounds such as tannins and bromelain. Field application on pak choi indicated that JLOF derived from tea dregs promoted the best plant growth among all groups. These findings suggest that Jakaba-based LOF formulated with tea dregs holds promise as an effective organic fertilizer and may be further developed for commercial use.

Keywords


Fermentation; fungal consortia; Jakaba; organic waste; plant growth

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Abatenh, E., Gizaw, B., Tsegaye, Z., & Wassie, M. (2017). The role of microorganisms inbBioremediation- A review. Environmental Biology, 1(1), 38–46.

Akbari, W. A., Fitrianingsih, Y., & Jati, D. R. (2018). Pemanfaatan limbah kulit pisang dan tanaman Mucuna bracteata sebagai pupuk kompos. Jurnal Teknologi Lingkungan Lahan Basah, 3(1), 1–10.

Ali, S., Asma, S. T., Nadeem, S. F., & Samar, M. (2018). Strategies and kinetics of industrial fermentation for the mass production of various primary and secondary metabolites from microbes. European Journal of Pharmaceutical and Medical Research, 5(6), 595–606.

Ambardini, S., Yanti, N. A., Mamangkey, J., Arsyat, E. Y., & Ardiansyah (2025). Biofungicides derived from indigenous microorganisms fermented on Tagetes erecta L. flowers for the control of anthracnose in chili pepper (Capsicum annuum L.). Plant Science Today, 12 (1), 1–9. https://doi.org/10.14719/pst.4130

Andraskar, J., Yadav, S., & Kapley, A. (2021). Challenges and control strategies of odor emission from composting operation. Applied Biochemistry Adn Biotechnology, 193, 2331–2356.

Arekemase, M. O., Omotosho, I. O., Agbabiaka, O. T., Ajide-Bamigboye, N. T., Lawal, A. K., Ahmed, T., & Oroju, J. O. (2020). Optimization of bacteria pectinolytic enzyme production using banana peel as substrate under submerged fermentation. Science World Journal, 15(1), 56-63.

Aruna, T. E. (2019). Production of value-added product from pineapple peels using solid state fermentation. Innovative Food Science and Emerging Technologies, 57, 102193. https://doi.org/10.1016/j.ifset.2019.102193

Calicioglu, O., Shreve, M. J., Richard, T. L., & Brennan, R. A. (2018). Effect of pH and temperature on microbial community structure and carboxylic acid yield during the acidogenic digestion of duckweed. Biotechnology for Biofuels, 11(275), 1–19. https://doi.org/10.1186/s13068-018-1278-6

Chew, K. W., Chia, S. R., Yen, H. W., Nomanbhay, S., Ho, Y. C., & Show, P. L. (2019). Transformation of biomass waste into sustainable organic fertilizers. Sustainability (Switzerland), 11(8), 1–19. https://doi.org/10.3390/su11082266

Czatzkowska, M., Harnisz, M., Korzeniewska, E., & Koniuszewska, I. (2020). Inhibitors of the methane fermentation process with particular emphasis on the microbiological aspect: A review. Energy Science and Engineering, 8(5), 1880–1897. https://doi.org/10.1002/ese3.609

Fadilah, R. A., Kurnia, M. D., & Putra, I. P. (2024). Jakaba undercover : Taxonomic riddle and potency in Indonesian agriculture. Caraka Tani: Journal of Sustainable Agriculture, 39(2), 411–423. https://doi.org/10.20961/carakatani.v39i2.89049

Fathi, A. (2022). Role of nitrogen (N) in plant growth, photosynthesis pigments, and N use efficiency : A review. Agrisost, 28, 1–8. https://doi.org/10.5281/zenodo.7143588

Guan, N., Li, J., Shin, H. dong, Du, G., Chen, J., & Liu, L. (2017). Microbial response to environmental stresses: from fundamental mechanisms to practical applications. Applied Microbiology and Biotechnology, 101(10), 3991–4008. https://doi.org/10.1007/s00253-017-8264-y

Hardie, D. G. (2018). Keeping the home fires burning: AMP-activated protein kinase. Journal of the Royal Society. Interface, 15(138), 20170774. https://doi.org/10.1098/rsif.2017.0774

Heiborn, D. heidler von, Reinmüller, J., Yurkov, A., Stehle, P., Moeller, R., & Lipski, A. (2023). Fungi under modified atmosphere—The effects of CO2 stress on cell membranes and description of new yeast Stenotrophomyces fumitolerans gen. nov., sp. nov. Journal of Fungi, 9(10). https://doi.org/10.3390/jof9101031

Hikal, W. M., Mahmoud, A. A., Ahl, H. A. H. S.-A., Bratovcic, A., Tkachenko, K. G., Kačániová, M., & Rodriguez, R. M. (2021). Pineapple (Ananas comosus L. Merr.), waste streams, characterisation and valorisation: An overview. Open Journal of Ecology, 11(09), 610–634. https://doi.org/10.4236/oje.2021.119039

Inarejos-García, A. M., Helbig, I., Klette, P., Weber, S., & Maeder, J. (2021). Authentication of commercial powdered tea extracts (Camellia sinensis L.) by gas chromatography. Food Science and Technology, 1(4), 596–604. https://doi.org/10.1021/acsfoodscitech.1c00003

Ismail, H. A., Richard, I., Ramaiya, S. D., Zakaria, M. H., & Lee, S. Y. (2023). Browning in relation to enzymatic activities and phytochemical content in terap peel (Artocarpus odoratissimus Blanco) during postharvest ripening. Horticulturae, 9(57), 1–15. https://doi.org/10.3390/horticulturae9010057

Ismail, M. A., Amin, M. A., Eid, A. M., Hassan, S. E. D., Mahgoub, H. A. M., Lashin, I., Abdelwahab, A. T., Azab, E., Gobouri, A. A., Elkelish, A., & Fouda, A. (2021). Comparative study between exogenously applied plant growth hormones versus metabolites of microbial endophytes as plant growth-promoting for phaseolus vulgaris l. Cells, 10(5), 1–26. https://doi.org/10.3390/cells10051059

Janˇciˇc, U., & Gorgieva, S. (2022). Bromelain and nisin: The natural antimicrobials potential in biomedicine. Pharmaceutics, 14(76), 1–39.

Kholifani, Y. I., Arbiwat, D., & Peniwiratri, L. (2020). The effects of mycorrhizae and organic matters application on soil p availability of limestone post mining and the growth of maize in Karangdawa Village, Tegal Regency. Proceeding International Conference on Green Agro-Industry, 4, 404-413.

Kibria, A. A., Kamrunnessa, Rahman, M. M., & Kar, A. (2019). Extraction and evaluation of phytochemicals from banana peels (Musa sapientum) and banana plants (Musa paradisiaca). Malaysian Journal of Halal Research, 2(1), 22–26. https://doi.org/10.2478/mjhr-2019-0005

Kurniawan, L., Maryudi, M., & Astuti, E. (2024). Utilization of tofu liquid waste as liquid organic fertilizer using the fermentation method with activator effective microorganisms 4 (EM-4): A review. Equilibrium Journal of Chemical Engineering, 8(1), 100–112. https://doi.org/10.20961/equilibrium.v8i1.84056

Li, L., Guo, X., Zhao, T., & Li, T. (2021). Green waste composting with bean dregs, tea residue, and biochar: Effects on organic matter degradation, humification and compost maturity. Environmental Technology and Innovation, 24(35), 101887. https://doi.org/10.1016/j.eti.2021.101887

Liu, L., Wang, S., Guo, X., Zhao, T., & Zhang, B. (2018). Succession and diversity of microorganisms and their association with physicochemical properties during green waste thermophilic composting. Waste Management, 73, 101–112. https://doi.org/10.1016/j.wasman.2017.12.026

Lu, Z., & Imlay, J. A. (2021). When anaerobes encounter oxygen: Mechanisms of oxygen toxicity, tolerance and defence. Nature Reviews Microbiology, 19(12), 774–785. https://doi.org/10.1038/s41579-021-00583-y

Maryana, Haryanto, D., & Lifia, R. C. (2023). Role of tea dregs liquid organic fertilizer and plant media composition in increasing tomato results (Lycopersicon esculentum Mill.). Journal Techno, 9(1), 1–10.

Minarni, M., & Riga, R. (2024). Phytochemical potentials and antibacterial activity of pineapple peel extract (Ananas Comosus L Merr) against Streptococcus mutans. Migration Letters, 7(57), 1833–1838.

Nadeem, F., Hanif, M. A., Majeed, M. I., & Mushtaq, Z. (2018). Role of macronutrients and micronutrients in the growth and development of plants and prevention of deleterious plant diseases - A comprehensive review. International Journal of Chemical and Biochemical Sciences, 13, 31–52.

Nanda, R. F., Rini, B., Syukrti, D., Thu, N. N. A., & Kasim, A. (2020). A review: Application of bromelain enzymes in animal food products. And. Int. J. Agric. Nat. Sci, 1(1), 33–44.

Ndese, T. V. P., Kamagi, D. D. W., Lawalata, H. J., Mokosuli, Y. S., & Taulu, M. L. S. (2024). The effect of biofertilizer (Jakaba) fertilizer on vegettive growth of chili plants (Capsicum annum L.). Advances in Tropical Biodiversity and Environmental Sciences, 8(1), 119–124. https://doi.org/10.24843/ATBES.2024.v08.i03.p01

Nitami, A. P. J., & Asngad, A. (2023). Quality of kirinyuh leaf liquid organic fertilizer and coconut water waste with pineapple skin bioactivator. International Conference on Biology Education, Natural Science, and Technology, 1(1), 122–132.

Orhan, T. Y., & Sahin, N. (2018). The impact of innovative teaching approaches on biotechnology knowledge and laboratory experiences of science teachers. Education Sciences, 8(4), 1-24. https://doi.org/10.3390/educsci8040213

Panpatte, D. G., Jhala, Y. K., Shelat, H. N., & Vyas, R. V. (2016). Pseudomonas fluorescens: A promising biocontrol agent and PGPR for sustainable agriculture. Microbial Inoculants in Sustainable Agriculture Productivity, 1, 257–270. https://doi.org/10.1007/978-81-322-2647-5

Pujiati, & Asngad, A. (2024). Efektivitas pupuk organik cair jerami padi dan ampas teh terhadap pertumbuhan dan kandungan kalsium tanaman selada (Lactuca sativa L.). Jurnal Pendidikan Biologi Dan Sains, 7(1), 284–293. https://doi.org/10.58351/2949-2041.2024.8.3.013

Rahman, K. M. A., & Zhang, D. (2018). Effects of fertilizer broadcasting on the excessive use of inorganic fertilizers and environmental sustainability. Sustainability (Switzerland), 10(3), 1–15. https://doi.org/10.3390/su10030759

Ranadheera, C. S., Mcconchie, R., Phan-thien, K., & Bell, T. (2017). Strategies for eliminating chicken manure odour in horticultural applications. Wordl’s Poultry Science Journal, 73(2), 365–378. https://doi.org/10.1017/S0043933917000083

Rashmi, I., Roy, T., Kartika, K. S., Pal, R., Coumar, V., Kala, S., & Shinoji, K. C. (2020). Organic and inorganic fertilizer contaminants in agriculture: Impact on soil and water resources. Contaminants in Agriculture: Sources, Impacts and Management, 3–41. https://doi.org/10.1007/978-3-030-41552-5

Risman, A. (2022). Pertumbuhan dan produksi tanaman cabai katokkon (Capsicum chinense Jacq) pada berbagai konsentrasi pupuk jakaba. [Skripsi]. Universitas Bosowa.

Rofi’i, M., Susanti, A., & Zuhria, S. A. (2021). The formulation’s technique using microbes to the speed decomposition of biomass fertilizers. AGARICUS: Advances Agriculture Science & Farming, 1(1), 28–36.

Salim, R., Nehvi, I. B., Mir, R. A., Tyagi, A., Ali, S., & Bhat, O. M. (2023). A review on anti-nutritional factors: Unraveling the natural gateways to human health. Frontuers in Nutrition, 10(1215873). https://doi.org/10.3389/fnut.2023.1215873

Samanta, S. (2020). Potential bioactive components and health promotional benefits of tea (Camellia sinensis). Journal of the American College of Nutrition, 41(1), 1–29. https://doi.org/10.1080/07315724.2020.1827082

Sari, R., Maryam, & Yumah, R. A. (2023). Penentuan C-Organik pada tanah untuk meningkatkan produktivitas tanaman dan keberlanjutan umur tanaman dengan metoda spektrofotometri UV VIS. Jurnal Teknologi Pertanian, 12(1), 11–19.

Sekaringsih, N. J., & Asngad, A. (2023). LOF kirinyuh leaves and liquid waste tempeh with pineapple peel bioactivator : Sensory and macronutrient content. International Conference on Biology Education, Natural Science, and Technology, 1(1), 335–347.

Sutikarini, S., Masulili, A., Suryani, R., Setiawan, S., & Mulyadi, M. (2023). Characteristics of pineapple waste as liquid organic fertilizer and its effect on ultisol soil fertility. International Journal of Multi Discipline Science, 6(1), 38. https://doi.org/10.26737/ij-mds.v6i1.3754.

Virginia, R., Wahyunigtyas, R.S., Silalahi, M., & Mamangkey, J. (2023). Pengaruh Limbah Organik Terhadap Pertumbuhan Kangkung Darat (Ipomoea reptans Poir) Dan Pemanfaatannya Sebagai Bahan Ajar. ORYZA Journal of Biology Education, 12 (2), 252-260. https://doi.org/10.33627/oz.v2i2.1337

Wang, J., Chen, Y., Wang, P., Li, Y. S., Wang, G., Liu, P., & Khan, A. (2018). Leaf gas exchange, phosphorus uptake, growth and yield responses of cotton cultivars to different phosphorus rates. Photosynthetica, 56(X), 1–8.

Yu, H., Xie, B., Khan, R., & Shen, G. (2019). The changes in carbon, nitrogen components and humic substances during organic-inorganic aerobic co-composting. Bioresource Technology, 271, 228–235. https://doi.org/10.1016/j.biortech.2018.09.088

Yu, T., & Chen, Y. (2019). Effects of elevated carbon dioxide on environmental microbes and its mechanisms: A review. Science of the Total Environment, 655, 865–879. https://doi.org/10.1016/j.scitotenv.2018.11.301

Yuan, H., & Zhu, N. (2016). Progress in inhibition mechanisms and process control of intermediates and by-products in sewage sludge anaerobic digestion. Renewable and Sustainable Energy Reviews, 58, 429–438. https://doi.org/10.1016/j.rser.2015.12.261

Yusminan, Walida, H., Harahap, F. S., & Mustamu, N. E. (2022). Comparison of Jakaba growth with the addition of organic matter in rice washing water. International Journal of Science and Environment (IJSE), 2(2), 74–78. https://doi.org/10.51601/ijse.v2i2.16

Zhu, J., Wang, J., Yuan, H., Ouyang, W., Li, J., Hua, J., & Jiang, Y. (2022). Effects of fermentation temperature and time on the color attributes and tea pigments of Yunnan Congou black tea. Foods, 11(1845), 1–15. https://doi.org/10.3390/foods11131845

Zou, F., Tan, C., Zhang, B., Wu, W., & Shang, N. (2022). The valorization of banana by-products: Nutritional compositon, bioactivities, applications, and future development. Foods, 11(20), 1–25. https://doi.org/10.3390/foods11203170




DOI: http://dx.doi.org/10.30821/biolokus.v8i1.4470

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